Cloud-Clone details STZ diabetic rat model for multi-organ research

Cloud-Clone Corp. outlined a standardized streptozotocin-induced SD rat model for diabetes research, describing how the protocol reliably produces hyperglycaemia and organ damage in heart, liver, kidney and lung tissue. The company says the workflow is meant to support drug screening, mechanism studies and other metabolic-disease research worldwide.

Why it matters: - Diabetes research depends on animal models that can reproduce both high blood sugar and the organ damage seen in humans. - A standardized model can help researchers compare drug effects, toxicity and disease mechanisms more consistently. - Cloud-Clone says its platform is designed to support model construction, sample processing and pathology work for metabolic-disease studies.

What happened: - Cloud-Clone Corp. published a detailed protocol for building a streptozotocin-induced diabetic SD rat model. - The model uses SPF-grade male SD rats that are 4-6 weeks old and weigh 180-200 g. - The protocol calls for six groups: normal control, model, positive-drug, low-dose test-drug, medium-dose test-drug and high-dose test-drug. - Each group includes 15 animals. - The experiment runs for 4 weeks. - The press release was issued from Huston, Texas, on August 25, 2026.

The details: - Streptozotocin selectively damages pancreatic islet β-cells and is used to trigger stable hyperglycaemia. - The protocol uses a single intraperitoneal dose of STZ at 55 mg/kg for the model group. - The normal control group receives an equal volume of blank sodium citrate buffer. - Rats are fasted for 12 hours before modeling, with water allowed. - Seven days after STZ administration, fasting blood glucose is measured. - Only rats with fasting blood glucose between 13.5 and 25 mmol/L enter the formal experiment. - Researchers monitor fasting blood glucose and body weight weekly after model creation. - At week 4, researchers collect blood and harvest epididymal fat, subcutaneous fat, skeletal muscle, white adipose tissue, liver, lung, heart and kidney tissue. - The protocol uses citric acid and trisodium citrate dihydrate to prepare a 0.1 mol/L sodium citrate buffer at pH 4.0. - STZ is prepared as a 10 mg/mL working solution, filtered through a 0.22 μm filter and kept light-shielded. - The company says the solution should be prepared fresh immediately before use. - Blood samples are left at room temperature for 2 hours, then centrifuged at 3000 r/min for 10 minutes at 4°C to isolate serum. - Serum is stored at -80°C for later analysis. - Statistical analysis uses SPSS, with data reported as mean ± standard deviation. - One-way ANOVA is used for multi-group comparisons, and Student’s t-test is used for two-group comparisons. - P < 0.05 is considered statistically significant.

Between the lines: - The protocol is built to produce not just hyperglycaemia, but also multi-organ complications that mirror broader diabetic disease burden. - The model produced reduced body weight, lethargy, slow response, coarse fur, hunched posture and polyuria. - Diabetic rats also showed higher heart and liver organ coefficients than controls. - Fasting blood glucose, triglycerides and total cholesterol rose in the model group, signaling disrupted glucose and lipid metabolism. - Heart tissue showed disorganized fibers, hypertrophic cardiomyocytes, inflammatory infiltration and fibroblast growth. - Lung tissue showed alveolar collapse, thickened bronchial walls, interstitial matrix expansion and inflammatory-cell infiltration. - Kidney tissue showed glomerular hypertrophy, thickened mesangial basement membrane and vacuolar degeneration of renal tubular epithelial cells. - Liver staining showed widespread lipid droplets and abnormal hepatic fat accumulation. - Cloud-Clone positions the workflow as useful for pathogenesis studies, hypoglycaemic drug screening, pharmacodynamic assessment and complication research.

What's next: - Researchers using the protocol can apply it to diabetes mechanism studies, intervention testing and organ-complication analysis. - Cloud-Clone says its standardized SPF animal platform can also support models for metabolic disorders, inflammation and oncology. - The company says it offers animal housing, model building, dosing, sample processing and pathological testing as part of a one-stop service. - Cloud-Clone directs readers to more information and lists social channels on LinkedIn, Instagram, TikTok, LinkedIn profile, Instagram profile, TikTok profile and X.

The bottom line: - Cloud-Clone is packaging a familiar STZ rat model as a standardized, reproducible tool for diabetes and multi-organ complication research.